Journal of East China Normal University(Natural Sc ›› 2010, Vol. 2010 ›› Issue (4): 92-102.

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Preparation of Grifola frondosa polysaccharide sulfatedderivatives and their inhibitory effects on HMEC migration

MAO Yi-rong, ZHANG Yi, ZHANG Hong-xia, WENG Liang, ZHANG Hong-feng   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2009-04-01 Revised:2009-08-01 Online:2010-07-25 Published:2010-07-25
  • Contact: ZHANG Hong-feng

Abstract: Polysaccharides from Grifola frondosa (GFP) were extracted by hot water and precipitated by 95% EtOH. GFP1-F,GFP1-M and GFP1-L were further purified by DEAE-cellulose and Sephadex G-100 subsequently from GFP. GFP1-F,GFP1-M and GFP1-L were glucan with molecular weight 1.09×10^5, 1.93×10^4 and 2.76×10^3 Da. GFP1-FS, GFP1-MS and GFP1-LS were polysaccharide sulfates obtained from GFP1-F,GFP1-M and GFP1-L with chlorosulfonic acidpyridine. The IR spectrum of GFP1-FS,GFP1-MS and GFP1-LS showed the characteristic absorptions of sulfate ester bond at 1 230 cm^-1and 810 cm^-1 . The 13C-NMR results indicated the modification mainly occurred at C-6 of the polysaccharide sulfates. GFP1-MS had the greatest sulfated degree with DS 1.07, while DSs of GFP1-FS and GFP1-LS were 0.66 and 0.61 respectively. The scratching assay suggested that 73.33%,34.17% and 67.21% cells migrated to scratching area compared with control after 24hour treatment of 1 000 μg/mL GFP1-FS, GFP1-MS and GFP1-LS respectively. So that all the three sulfated derivatives had inhibitory effects on HMEC migration, especially GFP1-MS had the strongest activity which may be related with its complicated branch structure.

Key words: glucan, sulfated derivative, HMEC, migration, Grifola frondosa, glucan, sulfated derivative, HMEC, migration

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