Diversity of soil microbes in a vertical forest

doi:10.3969/j.issn.1000-5641.2022.03.002

Abstract

Abstract:

In this study, we analyzed the diversity of microbes across 45 collected soil samples using meta-barcoding. The analysis showed similar α diversity of soil microbes in a vertical forest (VF) building and the surrounding green area, but a high level of differentiation in the microbe community composition and β diversity between these two types of habitats. The results indicated that a VF can accommodate a large number of microbe species and provided evidence for the contribution of VFs to the conservation of urban biodiversity.

Key words: urban eology, vertical forest, soil microbes, α diversity, β diversity

CLC Number:

Xianya XU, Stefano BOERI, Yibo XU. Diversity of soil microbes in a vertical forest[J]. Journal of East China Normal University(Natural Science), 2022, 2022(3): 8-16.

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苗木类型	植物名(拉丁名)
大型乔木 (高度 5.5 ~ 6 m)	土耳其榛(Corylus columa), 欧洲山毛榉(Fagus sylvatica), “旭日”美国皂荚(Gleditsia triacanthos"Sunburst"), 冬青栎(Quercus ilex)
中型乔木 (高度 3 ~ 5 m)	栓皮槭(Acer campestre), 棠棣(Amelanchier sinica), 美洲香槐(Cladrastis lutea), 花白蜡木(Fraxinus ornus), 金链树(Laburnum alpinum), 金蜂海棠(Malus"Golden Hornet"), 红宝石海棠(Malus"Red Jewel"), 欧洲野橄榄(Olea europaeavar. sylvestris), 波斯铁木(Parrotia persica), 大叶早樱(Prunus subhirtella), 十月樱(Prunus subhirtella"Autumnalis")
小型乔木和大型灌木 (高度 1.75 ~ 2 m)	草莓树(Arbutus unedo), 黄栌(Cotinus coggygria), 紫薇(Lagerstroemia indica), 星花木兰(Magnolia stellata), 桂花(Olea fragrans), 石榴(Punica granatum), 复兴樱桃(Prunus progressiflora), 红山紫茎(Stewartia pseudocamellia)

基因名称	引物 F 端序列	引物 R 端序列
16SrRNA	ACTCCTACGGGAGGCAGCAG	GGACTACHVGGGTWTCTAAT
ITS	CTTGGTCATTTAGAGGAAGTAA	GCTGCGTTCTTCATCGATGC
COI	GGWACWGGWTGAACWGTWTAYCCYCC	TANACYTCNGGRTGNCCRAARAAYCA

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