This research study focuses on Tettigoniidea and Gryllidea insects distributed across Shanghai Pujiang Country Park, with data collected twice a month from April to December of Year 2020 and 2021. The results show that 8 species of Tettigonioidea, 16 species of Grylloidea, and 1 species of Gryllotalpidae live in Shanghai Pujiang Country Park. The adult phase and voltinism in their life cycles, moreover, were found to be stable. Most Tettigoniidea and Gryllidea tend to overwinter in soil as diapause eggs, and a proportion of them overwinter as nymphs. The research suggests, furthermore, that using the calling songs of Tettigoniidea and Gryllidea can be a simple and effective way to carry out studies about phenology and ecology of singing insect.
In this work, micro-scale porous carboxyl polystyrene microspheres (PS) were prepared using seed polymerization. Quantum dots (QDs) were used as fluorescent molecules to synthesize QDs with different emission wavelengths and successfully loaded into porous microspheres to form fluorescent coding microspheres (QDs@PS). Subsequently, serum samples of patients with acute leukemia (AL) were detected, and the antigen in the serum was quantitatively analyzed using flow cytometry. Scanning electron microscope (SEM) and flow cytometry images showed that the microspheres were regular and uniform in size. Fluorescence microscopy showed that the QDs permeated uniformly into the whole microsphere. In addition, QDs@PS showed good fluorescence stability, no QD leakage was observed, and the QDs@PS maintained its fluorescence for a period of at least two weeks. The use of fluorescence spectroscopic analysis for the detection of human immunoglobulin G (IgG) showed that the carboxyl groups on the surface of fluorescent microspheres are beneficial for the efficient covalent binding of biological macromolecules, which can be used for sandwich immunosandwich reaction coupling with leukemic high expression antigen interleukin 6 (IL-6). Combined with serum samples from leukemia patients, the fluorescence of QDs was detected by flow cytometry, and the mean fluorescence intensity (MFI) was calculated to determine the content of IL-6 in the serum. These results indicate that the designed optically-encoded microcarrier can be successfully applied to high-throughput and multichannel biomolecular analysis and has great potential in blood disease detection and diagnosis.
In this research, we carried out a survey based on one fixed transect for Tettigonioidea and Grylloidea insects distributed in Tianmu Mountain from April to October of 2019. The results showed that there were 28 species of Tettigonioidea and 19 species of Grylloidea in Tianmu Mountain. Among them, six species of Tettigonioidea and eight species of Grylloidea were recorded in Tianmu Mountain for the first time. The insects became adults in August, September, and October. The insects distributed at lower altitudes tended to become adults earlier than those at higher altitudes. The number of species declined initially with increasing altitude, and subsequently increased. The Tettigonioidea species are distributed at various altitudes while Grylloidea species are primarily distributed at low altitudes. Because Truljalia tylacantha, Ruidocollaris truncatolobata, Goniogryllus punctatus and some other species are only distributed in a narrow scope at high altitudes, they can be used as indicator species for climate change in Tianmu Mountain.
Cricket fighting is a traditional Chinese game that has lasted for more than 1000 years. Before competitions, Xishuai grass (stimulant grass) is commonly used to hit a cricket’s head and antennae to prepare the insects for battle. In “Flora of China” and flora of Chinese provinces, Xishuai grass is referenced as the Chinese common name of Eleusine indica (L.) Gaertn. In “Flora of Beijing” and “Flora of Hebei”, Xishuai grass is referenced as the Chinese common name of the genus Eleusine. In this paper, however, we demonstrate that Xishuai grass should actually be Digitaria spp. Given the mistakes in the above flora references, this paper aims to clarify the correct classification for future reference.
In this study, the MS2 phages were used as an indicator microorganism to test the function of seven disinfectants with different components: hydrogen peroxide, ethanol, fermented lactic acid disinfectant, iodine disinfectant, quaternary ammonium disinfectant, chlorine-containing disinfectant, and peracetic acid disinfectant. Our results showed that the virus disinfection rate varied notably between the selected disinfectants. The iodine disinfectant exhibited the strongest disinfection effect, followed by the quaternary ammonium salt disinfectant and the peracetic acid disinfectant, while the disinfection effects of hydrogen peroxide, ethanol, and fermentation lactic acid disinfectant were inadequate. The test results provide a reference for the efficient utilization of various disinfectants to eliminate harmful microorganisms in the environment.
Plagiochila is one of the genera with the largest number of species among liverworts. A study was conducted to understand the diversity and distribution of the Plagiochila genus in Anhui Province. Based on the results of the study, there were 23 species of Plagiochila found in the region, of which seven species and one subspecies were first observed in Anhui Province. Floristic analysis showed that tropical Asian elements were the most abundant, accounting for 47.83%, and East Asian elements were the second most abundant, accounting for 34.78%. In the future, the accuracy of bryophyte identification can be further improved; meanwhile, the scope and depth of future investigations should be reinforced.
In this study, Gly→Ala was introduced into three chains of the heterotrimeric model (abc); seven mutants were subsequently constructed, and the local structure and global motion changes were analyzed. DSC results showed that the Tm value of the single point mutation was reduced by about 15°C, while the double and triple point mutations did not form triple helical structures. MD simulation trajectories were analyzed by ladder models; the results showed that the value of the step parameter changes near the mutation point, indicating an unfolding of the triple helix structure. An elastic function was introduced to quantify the degree of collagen structure change. It was found that the hydrogen bond energy was highly correlated with the structural deformation fraction ( $ R^2=0.76 $ ), indicating that the mutation not only destroyed the hydrogen bond force, but also resulted in changes in the bending and motion states of the molecule. This study, combined with calculations and experiments, helped quantify the effects of glycine mutation on the overall structure and movement pattern of collagen. Hence, the study provides a theoretical basis for clarifying the pathogenic mechanism of glycine mutation.
The Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC) databases were used to collect RNA sequence information from patients with hepatocellular carcinoma (HCC). The key genes involved in the immune response mechanism to HCC were screened using the non-negative matrix factorization (NMF) clustering method and weighted gene co-expression network analysis (WGCNA). Prognostic gene models were constructed using the least absolute shrinkage and selection operator (LASSO) regression analysis, and biological functions were analyzed using gene set enrichment analysis (GSEA). Subsequently, to assess the immune infiltration and the related functional differences between the patients in two different risk groups , we used single-sample gene set enrichment analysis (ssGSEA). We constructed column line graphs in combination with independent risk factors to predict overall patient survival time using the “RMS” package in R. Finally, preliminary clinical validation was performed using the Human Protein Atlas (HPA) database with real-time quantitative fluorescent PCR (RT-qPCR). In conclusion, we integrated the clinical characteristics of patients based on risk scores to construct a verifiable and reproducible column line chart, providing a reliable reference for the precise treatment of patients in clinical oncology.
The aim of this study was to update the bryophyte list of Dajinshan Island and provide the scientific basic data for in situ conservation. Based on five field investigations on the island, 67 species belonging to 38 genera in 20 families are reported herein. Compared with historical data for Dajinshan Island, 23 species are newly recorded in the island. Of these, 13 species are newly recorded in Shanghai. One epiphyllous liverwort species, Cololejeunea raduliloba Steph., is newly reported on Dajinshan Island. Taking into account climate change and the physiological and ecological characteristics of bryophytes, the changes in bryophyte species composition on Dajinshan Island are discussed. Our results highlight the importance of timely updating of a regional checklist, when conserving bryophyte biodiversity.
To ascertain the status and promote the utilization and sharing of type specimens in Herbarium of Shanghai Natural History Museum (SHM), the collecting information of normal specimens in SHM with type specimens in specimens of plant resource sharing platform and journal of plant taxonomy were compared, 418 type specimens were confirmed. There are 239 species belonging to 147 genera in 69 families, including 390 type specimens newly discovered. The quantity, type, species, dominant groups, collectiion location, collection time, and the name type specimen collector were collected and analysed in the herbarium.
This study was conducted to evaluate the effects of an 8-week altitude training on erythropoiesis, iron metabolism, and aerobic capacity in trained rowers. Twenty-eight trained rowers were divided into the altitude training (AT) and sea-level training (ST) groups. During the 8-week training camp, the training plan and load were similar in both groups. VO2peak, red blood cell count (RBC), reticulocyte% (RET%), hemoglobin (Hgb), and concentrations of serum erythroferrone (ERFE), ferritin (FER), and soluble transferrin receptor (sTfR) were measured before and after the 8-week training camp. It found that (1) compared with the pre-value, VO2peak and VO2peak to body mass (RVO2peak) increased significantly after the 8-week training in the AT group. No obvious differences in VO2peak and RVO2peak were observed in the ST group. The changes in VO2peak and RVO2peak between the two groups were significant (+9.41% vs +3.03%, p<0.05; +12.83% vs +0.80%, p<0.01). (2) After the 8-week training, the RBC, Hgb, and hematocrit (HCT) increased in the AT group but no statistical difference in the ST group. Changes in Hgb and HCT between the two groups were significant (+4.95% vs –3.21%, p<0.01; +6.48% vs –1.57%, p<0.01). A significant trend in RBC count change was observed between the two groups (+3.19% vs –3.61%, p=0.061). Compared with the pre-test values, no significant changes in RET% and reticulocyte hemoglobin equivalent (RET-He) were found in either groups after the 8-week training. The AT group showed significantly increased of low fluorescent reticulocyte (LFR) and reticulocyte production index (RPI) and significantly decreased medium fluorescent reticulocyte (MFR) and high fluorescent reticulocyte (HFR). There were no significant differences in RET%, RET-He, LFR, MFR, HFR, and IRF (immature reticulocyte fraction) in both groups. However, changes in RPI between both groups after the training camp was significant (+30.60% vs –4.52%, p<0.05). (3) In the AT group, no remarkable changes in serum ERFE, a significant decrease in serum FER, and an increase in serum sTfR and sTfR/lg(FER) levels were observed after of the 8-week training. In the ST group, there were no statistical changes in serum FER, sTfR, and sTfR/lg(FER) and significantly increased serum ERFE. Changes in serum FER, ERFE, sTfR, and sTfR/lg(FER) levels differed significantly between both groups (+17.99% vs +121.31%, p<0.05; –36.16% vs –2.96%, p<0.05; +82.77% vs –8.87%, p<0.05; +108.40% vs –6.96%, p<0.05). (4) There was a significantly positive association between the change in VO2peak and serum sTfR levels and ratio of sTfR to lg(FER) after the 8-week training. Therefore , eight weeks of AT appears to be more effective than ST in improving the oxygen delivery capacity of the blood and aerobic capacity in trained rowers. In the later stage of the 8-week AT, erythropoiesis remained active. Serum sTfR levels may be important in improving aerobic performance.
中国综合性科技类核心期刊(北大核心)
