From the perspective of ecology and by using the theory of ecological niche, phytoplankton functional group has a better description about the relationship between phytoplankton and their environment, and provides a more accurate basis for freshwater system research about the composition of phytoplankton and water monitoring. This research of phytoplankton functional group composition and influence factor is carried out in spring and summer in 2015 in Shanghai Dianshan Lake, and phytoplankton were collected and respectively in the function of the corresponding group. The results for spring function group (20 in total) B, C, D, E, F, G, H1, J, Lo, M, MP, P, TB, TC, W1, W2, X1, X2, X3, and Z. Summer function group (17 in total) includes B, C, D, F, G, J, Lo, M, MP, TB, TC, W1, W2, X1, X2, X3, and Z. Advantage function group for the spring is X1, X2 and, Lo, J; summer advantage function group is X2, X1, P, Lo. Through the analysis of
the RDA, the main factor which has a great influence on phytoplankton advantages is Kd, T, DO, and TP.