Journal of East China Normal University(Natural Sc ›› 2018, Vol. 2018 ›› Issue (6): 97-104.doi: 10.3969/j.issn.1000-5641.2018.06.012

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Analysis of microbial community composition from media used to strengthen total nitrogen removal from an urban river

HAN Jing1,2, XU Yi-wen1,2, HE Yan1,2, HUANG Min-sheng1,2   

  1. 1. School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China;
    2. Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai 200241, China
  • Received:2018-07-16 Online:2018-11-25 Published:2018-12-01

Abstract: We compared the removal rate of total nitrogen in urban rivers with three kinds of media (biochar, felt, and non-woven fabric). After 84 days in operation, it was found that non-woven fabric increased the removal rate of total nitrogen by 23.4% over the control group; felt increased the removal rate of total nitrogen by 13.9% over the control group; and biochar had a poor effect on total nitrogen removal. The microbial diversity and community composition of different media-enhanced groups were then systematically explored; the results showed that non-woven fabric can significantly increase microbial diversity and abundance. Moreover, proteobacteria, which is related to nitrogen conversion, was found to be significantly higher in the non-woven fabric group than the felt and biochar groups, especially the abundance of denitrifying bacteria (Azospirillum, Thiobacillus, and Azoarcus). The abundance of methanogen, which is in competition with the denitrifying bacteria Syntrophorhabdus, was found to be lower in non-woven fabric than in the control and biochar groups. The microbial flora structure of the felt group showed a similar trend to that of the non-woven fabric group, but the abundance of denitrifying bacteria was lower. The microbial community composition of the biochar group was the most similar to that of the control group. It is speculated that non-woven fabric has better biocompatibility than other groups; this is beneficial for biofilm formation and thereby promotes the growth of denitrifying bacteria. In a comprehensive comparison, non-woven media materials are more conducive to the removal of total nitrogen in urban rivers.

Key words: media, urban river, total nitrogen reduction, microbial action

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