Characteristics of dissolved organic matter and its effects on denitrification in urban river sediments

doi:10.3969/j.issn.1000-5641.2021.04.006

Abstract

Abstract:

Understanding the impact of dissolved organic matter (DOM) on the denitrification process is critical to addressing the challenges associated with nitrogen removal in urban river treatment. In this paper, we show that DOM in urban rivers are mainly comprised of small-molecule fulvic acids. The humic acid content and aromaticity of the DOM, moreover, were found to be low. Compared with the control case, DOM can promote the denitrification process; specifically, the removal efficiency of TN and NO₃^–-N in the DOM-added group increased by 7.24% ± 0.36% and 23.52% ± 1.17%, respectively. DOM with an acetate group had an even better effect on the removal of TN and NO₃^–-N, reaching 74.48% ± 1.29% and 98.62% ± 0.07%, respectively. Microbiological analysis showed that the DOM-added group can significantly increase the diversity and richness of the bacteria community compared with the control case. However, the relative abundance of the heterotrophic denitrifiers Pseudomonas and Brevundimonas as well as the nirK-type denitrifier Paracoccus in the DOM-added group was less than that of the DOM with an acetate group. Additionally, a relatively high concentration of NH₄⁺-N (> 3.7 mg/L) was observed in the DOM-added group. The addition of DOM can significantly increase the relative abundance of Anaeromyxobacter related to dissimilatory nitrate reduction to ammonium (DNRA) functional genes. It is speculated that DOM promotes the denitrification process and induces the DNRA process simultaneously.

Key words: urban river, dissolved organic matter, denitrification, fulvic acid, dissimilatory nitrate reduction to ammonium

CLC Number:

X522

Rui WENG, Zheng WEI, Yanmei YANG, Jing HAN, Yan HE, Minsheng HUANG. Characteristics of dissolved organic matter and its effects on denitrification in urban river sediments[J]. Journal of East China Normal University(Natural Science), 2021, 2021(4): 46-54.

Figures/Tables 7

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名称	引物	序列	参考文献
细菌16S rRNA	338F	5′-ACTCCTACGGGAGGCAGCAG-3′	[7]
细菌16S rRNA	806R	5′-GGACTACNNGGGTATCTAAT-3′	[7]
反硝化nirK	nirK-876	5′-ATCATGGTSCTGCCGCG-3′	[8]
反硝化nirK	nirK-1040	5′-GCCTCGATCAGRTTGTGGTT-3′	[8]
异化硝酸盐还原nrfA	nrfA2aw	5′-CARTGYCAYGTBGARTA-3′	[9]
异化硝酸盐还原nrfA	nrfAR1	5′-TWNGGCATRTGRCARTC-3′	[9]

菌属	功能	菌属丰度/%			参考文献
菌属	功能	空白	DOM	DOM协同乙酸盐	参考文献
Thiobacillus (硫杆菌属)	硫自养反硝化	12.69	17.76	0.17	[23]
Pseudomonas (假单胞菌属)	异养反硝化	0.5	5.43	13.15	[24]
Thauera (陶厄氏菌属)	异养反硝化	8.8	0.8	0.06	[25-26]
Thermomonas (热单胞菌属)	硫自养反硝化	0.81	5.67	0.47	[27]
Brevundimonas (短波单胞菌属)	异养反硝化, 脱氮除磷	0.41	2.87	8.15	[28]

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