Journal of East China Normal University(Natural Sc ›› 2019, Vol. 2019 ›› Issue (4): 156-164.doi: 10.3969/j.issn.1000-5641.2019.04.015

• Ecological and Environmental Sciences • Previous Articles     Next Articles

Effect of sulfide on the denitrification potential of sediment in black-odor rivers

WANG Shan1,2, ZHU Jin1,2, HE Yan1,2, HUANG Min-sheng1,2, ZHOU Yun-chang1,2   

  1. 1. School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China;
    2. Shanghai Key Laboratory of Urbanization Ecological Restoration, East China Normal University, Shanghai 200241, China
  • Received:2018-07-07 Online:2019-07-25 Published:2019-07-18

Abstract: This study aimed to explore the effect of sulfide on sediment denitrification in different concentrations, analyze the response of bacteria, including denitrifying and sulfate-reducing bacteria, and provide a theoretical basis and technical support for improving the denitrification process of sediment in black-odor rivers. The results showed that a low concentration of sulfides (8 mg·L-1) didn't have a significant effect. In constrast, a moderate concentration of sulfides (40 mg·L-1, 64 mg·L-1) promoted the denitrification process; in this range the higher the concentration of sulfides, the faster the rate of nitrate degradation. When the sulfide concentration rises to 96 mg·L-1 and above, reductive sulfur inhibits denitrification, and the higher the concentration, the more obvious the inhibition. After a period of denitrification of the culture, the bacterial diversity is mainly composed of Proteobacteria, Chlofloflexi, and Bacillus. As the same time, the total number of bacteria in the sediment increased; the abundance ratio of nirS to 16S rRNA gene and dsrB to 16S rRNA gene were 1.42% and 0.05%, respectively. Compared with the original sediment (0.15%, 0.19%), the denitrifying bacteria increased significantly, but the number of sulfate-reducing bacteria decreased.

Key words: black-odor rivers, reductive sulfur, denitrification, sediment

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