Effect of sulfide on the denitrification potential of sediment in black-odor rivers

doi:10.3969/j.issn.1000-5641.2019.04.015

Abstract

Abstract: This study aimed to explore the effect of sulfide on sediment denitrification in different concentrations, analyze the response of bacteria, including denitrifying and sulfate-reducing bacteria, and provide a theoretical basis and technical support for improving the denitrification process of sediment in black-odor rivers. The results showed that a low concentration of sulfides (8 mg·L^-1) didn't have a significant effect. In constrast, a moderate concentration of sulfides (40 mg·L^-1, 64 mg·L^-1) promoted the denitrification process; in this range the higher the concentration of sulfides, the faster the rate of nitrate degradation. When the sulfide concentration rises to 96 mg·L^-1 and above, reductive sulfur inhibits denitrification, and the higher the concentration, the more obvious the inhibition. After a period of denitrification of the culture, the bacterial diversity is mainly composed of Proteobacteria, Chlofloflexi, and Bacillus. As the same time, the total number of bacteria in the sediment increased; the abundance ratio of nirS to 16S rRNA gene and dsrB to 16S rRNA gene were 1.42% and 0.05%, respectively. Compared with the original sediment (0.15%, 0.19%), the denitrifying bacteria increased significantly, but the number of sulfate-reducing bacteria decreased.

Key words: black-odor rivers, reductive sulfur, denitrification, sediment

CLC Number:

X522

WANG Shan, ZHU Jin, HE Yan, HUANG Min-sheng, ZHOU Yun-chang. Effect of sulfide on the denitrification potential of sediment in black-odor rivers[J]. Journal of East China Normal University(Natural Sc, 2019, 2019(4): 156-164.

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