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Toxicity effects of oil and dispersant on sea urchin(Hemicentrotus pulcherrimus)
Lv Furong;;XIONG Deqi
2009, 2009 (3):
7-16.
The acute toxic effects of No.0 diesel oil wateraccomodated fractions(WAFs) and dispersed oil (disWAFs) on sea urchin were studied, and the response of antioxidase(superoxide dismutase, SOD; catalase, CAT; glutathione Stransferase,GST) in digestive gland to disWAFs were tested. Hemicentrotus pulcherrimus was exposed to different concentrations of disWAFs (0.5,1,2 mg/L) for 13 days, and then transferred into clean sea water for 11d restoring experiment. The results showed that the disWAFs was more toxic, with 24, 48, 72, 96h LC50 values of 11.7,9.1,7.4 and 5.1 mg/L respectively. The activity of antioxidase was statistically significant doserelated effect in different disWAFs concentrations. Under the same doseconcentration exposure, SOD, CAT and GST activities increased first and then decreased with the prolonged exposure time. This trend was most significant in GST activity. The activities of SOD, CAT and GST of all concentration groups recovered to the corresponding control level after recovery experiment. According to the data, SOD, CAT and GST can be taken as biomarkers to monitor benthic marine oil pollution and provide.
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