Decreased klotho expression and elevated blood phosphate level in presenilin PS1/PS2 conditional double knockout mice

Abstract

Abstract: In this study, we investigated the expression of klotho, an anti-aging gene, and its involvement of premature aging phenotypes of presenilin 1/presenilin 2 conditional double knockout (PS cDKO) mice. The results showed that renal klotho level was significantly decreased in 12-month-old female PS cDKO mice when compared with control mice. Meanwhile, significant elevations of plasma phosphorus but slight reduction of calcium and almost no sign of renal dysfunction were observed. In PS cDKO brains, down-regulated klotho level was observed as early as 3 months of age. These findings indicate a possible role of klotho in the disorders of PS cDKO mice. The declined klotho expression may be responsible for the excess blood phosphate, but it seems that such a moderate decline in klotho level and impaired phosphate metabolism were not sufficient to elicit renal dysfunction in PS cDKO mice. However, as klotho is an anti-oxidative and anti-inflammation factor, klotho decline may contribute to the increased oxidative stress and inflammation in PS cDKO mice.

Key words: klotho, PS cDKO mice, phosphate, calcium, aging

CLC Number:

Q786

WANG Dong， ZHAI Wen-zhu， SU Jing-jing， MENG Bo，ZHAO Zheng， HU Jin-feng. Decreased klotho expression and elevated blood phosphate level in presenilin PS1/PS2 conditional double knockout mice[J]. Journal of East China Normal University(Natural Sc, 2013, 2013(2): 75-83, 91.

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